Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------MQKFMNGWVNGFIGVAIFAGSLPATRVAVTGFEPGFLTAARAVIAGVLGLILIFLLKEKKPAKQDWWPLAIVALGVVVGFPLFTALALQYMNAAHSIVFVSLLPLATA---IFAVVRGGEKPNLFFWIFAVLGSLVVFTYMFFL--SGEASFGIGDLYMLIAIILCGFGYAE-----------GGVLSKKIGGWQVICWALILALPIM---LLATLFYMPVSFQD------------VSTSAVAGLVYVSLFSMLIGFFFWYKGLAQGGIAAISQ---------LQLLQPLMGLAIAALLLHEHVSWSMLMVTAVTILCVAAAKKFA 3DZO Chain:A ((13-382)) NLYFQGFRGTDPGDVVIEELFNRIPQANVRTTSEYAADSLVSTSLWNTGQPFRVESELGERPRTLVRGTVLGQEDPYAYLEATDQETGE------SFEVHVPYFTERAIKQMKEEVLRLRLLRGIKNQKQAKVHLRFIFPFDLVKDPQVLSRFFLYPRMQSNLQTFGEVLLSHSSTHKSLVHHARLQLTLQVIRLLASLHHYGLVHTYLRPVDIVLDQRGGVFLTGFEHLVRDGASAVSPIGRGFAPPETTAERMLPFGQHHPTLMTFAFDTWTLGLAIYWIWCADLPNTDDAALGGSEWIFRSCKNIPQPVRALLEGFLRYPKEDRLLPLQAM-ETPEYEQLRTELSAALPLYQ

General information: TITO was launched using: RESULT: Template: 3DZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 -135452 -100.56 -483.76 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -100.56 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_3DZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DZO-query.scw PDB file : Tito_Scwrl_3DZO.pdb: