Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCNKASSDLTDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKKIALTDLYFPQLGIHIEVDEGHHFLRNSKMEYSLNQIDEPLYSISQTESDAMREEDIISITGHKIFRVNVFKNQEGQPQNLENIHQQIDKIIEEIKTAKNKLIEASTFKEWNIETEYNPQTYIDLGRISLADNVVLKTTKDVCNCFGYSYKNYQRGGALHPYKKDTLIWFPRLYENKDWINTISPDGLTITEKSTDETITLKKLEEWKNGPQKRIVFARVKDNLSSRAMYRFMGLYEFQKADLKDGAVWKRVKCEVQTYSPKETKC 4PAR Chain:C ((4-317)) ---KASSDLTDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKKIALTDLYFPQLGIHIEVDEGHHFLRNSKMEYSLNQIDEPLYSISQTESDAMREEDIISITGHKIFRVNVFKNQEGQPQNLENIHQQIDKIIEEIKTAKNKLIEASTFKEWNIETEYNPQTYIDLGRISLADNVVLKTTKDVCNCFGYSYKNYQRGGALHPYKKDTLIWFPRLYENKDWINTISPDGLTITEKSTDETITLKKLEEWKNGPQKRIVFARVKDNLSSRAMYRFMGLYEFQKADLKDGAVWKRVKSEVQTYSPK----

General information: TITO was launched using: RESULT: Template: 4PAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1505 -109675 -72.87 -349.28 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.99 3D Compatibility (PKB) : -72.87 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_4PAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PAR-query.scw PDB file : Tito_Scwrl_4PAR.pdb: