Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIEIRTDKNIHNSERLITYVRAELTQEFQRHSERITHFSVHFSDENGDKGGDKDIHCMIEARPSGLKPVAVHHKAGNIDASIHGAIEKLKRSLEHTFEKKEHPRGGQPEFIDDEV 4HEI Chain:A ((1-92)) MQINIQG-HHIDLTDSMQDYVHSKFD-KLERFFDHINHVQVILRVEKL------RQIAEATLHVNQ-AEIHAHADDENMYAAIDSLVDKLVRQLNKHKEKL---------------

General information: TITO was launched using: RESULT: Template: 4HEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 394 -62734 -159.22 -681.89 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -159.22 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.672

(partial model without unconserved sides chains): PDB file : Tito_4HEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HEI-query.scw PDB file : Tito_Scwrl_4HEI.pdb: