Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITPMYVCLCRGITDQDIKDAIENGAESYREIRDLLDLGTCC--------GR-CAPEARAIISEELAEIAARISVAA 2HU9 Chain:A ((69-127)) --EEPKPVCYCNRVTEKMLLEAAEKFGK--EKAVEITGAGKGKWCVVTNPSGRCCHWHLERLG---------------

General information: TITO was launched using: RESULT: Template: 2HU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 151 -10806 -71.56 -216.12 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -71.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2HU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HU9-query.scw PDB file : Tito_Scwrl_2HU9.pdb: