Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHAFAEKTGVDLNYQKRLAPLDGFESSMRSFFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTLWMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVIANRTLARAEQLVDDLKTAVPQAQLQAISLNDLEGDFDIVINATSASLSGDALQLPEKLKF------KYAYEMAYGKPSSFLDQAKQRNVPYAEGFGMLVGQAIEAFSIWNGVRPQLKDFL 2HK7 Chain:A ((6-252)) -TQLYGVIGFPVKHSLSPVFQNALIRYAGLNAVYLAFEINPEELKKAFEGFKALKVKGINVTVPFKEEIIPLLDYVEDTAKEIGAVNTVKFENGKAYGYNTDWIGFLKSLKSLIPEVKEKSILVLGAGGASRAVIYALVKEGA-KVFLWNRTKEKAIKLAQKFPLEVVNSPEEVID------KVQVIVNTTSVGLKDED---PEIFNYDLIKKDHVVVDIIY-KETKLLKKAKEKGAKLLDGLPMLLWQGIEAFKIWNG---------

General information: TITO was launched using: RESULT: Template: 2HK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 -123245 -92.18 -511.39 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -92.18 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2HK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HK7-query.scw PDB file : Tito_Scwrl_2HK7.pdb: