Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRQQIDVLVKQMNIDTAKGEVDARVQQIVVRLLGDLFQAIEDLDIQPSEVWKGLEYFTDAGQANELGLLAAGLGLEHYLDLRADEADAKAGMTGGTPRTIEGPLYVAGAPESVGFAHMDDGTETGKIDTLIIEGTVTDTDGNIIENAKVEVWHANSLGNYSFFDKS--QSDFNLRRTIFTDADGKYVALTTMPVGYGCPPEGTTQALLNKLGRHGNRPSHVHYFVSAPGYRKLTTQFNIEGDEYLWDDFAFATRDGLVATAVDVTDPAEIQRRGLD-HAFKHITFNIELVKEATAAPSTEVERRRASA 5VXT Chain:A ((41-323)) ----------------------NARTQQIVHRLLGDLFKAIDDLDITPDEVWAGVNYLNKLGQDGEAALLAAGLGLEKYLDIRMDAEDEAIGLDGGTPRTIEGPLYVAGAPVRDGVAKIDLDADEGA-GPLVIHGTVTGLDGKPVAGALVECWHANSHGFYSHFDPTGKQSDFNLRGAVKTGADGKYEFRTLMPVGYGCPPQGATQQLLDRLGRHGNRPAHVHFFVTSDGHRKLTTQFNIEGDPLIWDDFAYATREELIPP---VTAKAGGAALGLKADAYQDIEFNFVLTPRVEGKDNQIVERLRASA

General information: TITO was launched using: RESULT: Template: 5VXT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1346 -10704 -7.95 -38.23 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -7.95 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_5VXT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VXT-query.scw PDB file : Tito_Scwrl_5VXT.pdb: