Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQQLVFKSKNALTDLTAIPDFVSYKSRVESDGGVIYDQQAVLDVLQFIFQNSIPETDILSATSAAWGVKFDPVTKVITKLYNLFN-PAGDIIIQNGTMNAIHTTEVDGKPSLYAGGTSTLYGYSSGKFAIANPIAHTIHHVPARSGYGASALLFPLQTLFNKEGFDASTDPAKVSTDYVAVDQRLTRNATTNNDPNTWNEAHRFWGASVGVDGNLNGSRIGYASNALTTGTFIYKDGAQSNSNSTVPNIKPTQQADQRLYLMSNFTVAGVRGNYYLGYIFENWLLNNGTDSIAKALSLRAKTKYR 4NN9 Chain:A ((131-230)) ------------------------------------------------------------------------PVTEINTWARNILRTQESECVCHNGVCPVVFT---DG--SATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSCYGERAEI----TCTCRDNWQGSNRPV-IRIDPVAM----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4NN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 424 -2903 -6.85 -29.32 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -6.85 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_4NN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NN9-query.scw PDB file : Tito_Scwrl_4NN9.pdb: