Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLISLPKQILQCGQLQLDLSQPH-VMGILNVTPDSFSDGGKHNQLDQAVDHALSMIEQGATIIDIGGESTRPGASEVAVEEEVRRVVPVVEALSHH-NVILSIDTSQPEVIRAAKAAGAHIWNDV-RALTGPNALKTAVELDIPVVIMHMRGEPTTMNQLDQYTDVTLDVMQELQQRIDEALAAGVKKHNIIVDPGFGFAKNAQQNLKLLKEFWKLNELGYPILSGLSRKRFIGEALQGAPADQRAVGSVTGHLLSIQQGASIVRAHDVKEMHEAILVWKAMQQA 1TWZ Chain:A ((27-291)) ----------LRCGEYTLNLNEKTLIMGILNVTPDSFSDGGSYNEVDAAVRHAKEMRDEGAHIIDIGGES-------VSVEEEIKRVVPMIQAVSKEVKLPISIDTYKAEVAKQAIEAGAHIINDIWGAKAEPKIAEVAAHYDVPIILMHNR---DNMN----YRNLMADMIADLYDSIKIAKDAGVRDENIILDPGIGFAKTPEQNLEAMRNLEQLNVLGYPVLLGTSRKSFIGHVLD-LPVEERLEGTGATVCLGIEKGCEFVRVHDVKEMSRMAKMMDAM---

General information: TITO was launched using: RESULT: Template: 1TWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1450 4475 3.09 17.55 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 3.09 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_1TWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TWZ-query.scw PDB file : Tito_Scwrl_1TWZ.pdb: