Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILVIADHNNQTLNGATLNVVAAAQ--KIGGDITVLVAGSGAQAVADAAA-KVAGVSKVLLADNAAY---ANQLAENVAGLVADLAKGYKYVLAASTTTGKNILPRVAALLDVSMITDIISVESANTFKRPIYAG-NAIATVQSD---EAIIVGTVRGTAFDPVAAEGGSAAVETVGEVKDAGV-SKFVSEEIVKL-DRPELTAARIVVSGGRGVGSGENYHKVLDPLADKLGAAQGASRAAVDAGFVPNDFQVGQTGKIV-APELYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPINSVADYWLVGDLNAVVPELVSKL 1O97 Chain:D ((3-318)) --ILVIAEHRRNDLRPVSLELIGAANGLKKSGEDKVVVAVIGSQADAFVPALSVNGVDELVVVKGSSIDFDPDVFEASVSALIA--AHNPSVVLLPHSVDSLGYASSLASKTGYGFATDVYIVEYQGDELVATRGGYNQKVNVEVDFPGKSTVVLTIRPSVFKPL--EGAGSPV--VSNVDAPSVQSRSQNKDYVEVG-DIDITTVDFIMSIGRGIGEETNVEQFRE-LADEAGATLCCSRPIADAGWLPKSRQVGQSGKVVGSCKLYVAMGISGSIQHMAGMKHVPTIIAVNTDPGASIFTIAKYGIVADIFDIEEELKAQL

General information: TITO was launched using: RESULT: Template: 1O97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1622 -1445 -0.89 -4.82 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -0.89 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_1O97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O97-query.scw PDB file : Tito_Scwrl_1O97.pdb: