Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAMLPEWTTACPDWEERIVAKKSLMPCEPLFPQVADVAERIFKELILVDVMGSPKMGDVTLDWVIEFVRAIFGSYNPNTKRRLIREFFLLISKKNTKSTIAAGVMLVALLLNDRLSAELIILAPTKEVAD-NSFNPIRDFIRADEELSAMINVSEHTKTVTHLGTGATLKVIAAE--SNAAAGKK-ASIILIDEVWLFGKRANAESMFREAKGGLASRPEGCVIYLSTMSDEVPCGVFKQLLDYARDVRDGIKEDKGFLPLIYEFPKRLVEAGEHLKPEHFYITNPNLGASVDLEYLISEFKKVKDAGEESLRDFLAKHLNIEIGMNLRANRWAGAEYWNKQKHVFGLDQLIEQSDVITIGIDGGGL-DDLLGYAVLGRLKKDPRVWWLWNHAWANKIALERRKENIPKYDDFKLEGSLTVVDRVGDDIDQLAA-IAKKVYDSGKLN-KIGLDPLG-LGGLLDGLLEA--GIPEESMFAVPQG---------------------YK-LMSYILTTERKLAEGN---------------------LYHAGQQLMTWAAGNARVVMVGN-GMRI-TKQ---E-SGVGKIDPLIATFNAVALMSMNPEPTNKEYNVFFV 4IEE Chain:A ((35-476)) ------------------------------------------------------------------------------------HRYKVAKGGRGSGKSWAIARLLVEAARRQ--P-VRILCARELQNSISDSVIRLLEDTIER-EGYSAEFE--IQRSMIRHLGTNAEFMFYGIKNNPTKIKSLEGIDICWVEEAEA----VT-KESWDILIPTIRK-PFSEIWVSFNPKNI--LDDTYQRFVVNP--------PDDICL--LTVNY---------------TDNPHFPEVL-RLEMEECKR----RNPT---LYRHIWLGEPVSAS--DMAIIKREWLEAATDAHK-KLGWKAKGAVVSAHDPSDTGPDAKGYASRHG-----SVVKRI-AEG----------------------------L--LMDINEGADWATSLAIE--DGADHYLWDGDGVGAGLRRQTTEAFSGKK-ITATMFKGSESPFDEDAPYQANVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLSFDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCMHCPALV-------------

General information: TITO was launched using: RESULT: Template: 4IEE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1753 -1586 -0.90 -4.26 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -0.90 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_4IEE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IEE-query.scw PDB file : Tito_Scwrl_4IEE.pdb: