Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRPTVLCFSGLDPSGGAGLQADIEAIGQSGAHAAIACTALTIQN-----SQQVFGSEATSKELLLAQANAVVGDLPIKCVKSGMLGTTDNIAALAEFLRAHPDYQYVLDPVLVANSGGSL--GDQATLVKAFVELILLATLITPNTVELRALTG------VTDLDQATQKLFEMGAKAVLVKGG----HEDTPDFIKNSLYIDGELAASSTCPRLEGEY-HGSGCSLASFIAGRLALGDSLKIAVQHAETWLFGVLKNAETPVPNGQKIPKRF 2I5B Chain:A ((7-250)) -----LTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVF---PIDTDTIRAQLATITDGIGVDAMKTGMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLYPEHAQALRE--QLAPLATVITPNLFEASQLSGMDELKTVDDMIEAAKKIHALGAQYVVITGGGKLKHEKAVDV----LY-DGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAKEFITAAIKES--------------

General information: TITO was launched using: RESULT: Template: 2I5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1235 -21933 -17.76 -97.05 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -17.76 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_2I5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I5B-query.scw PDB file : Tito_Scwrl_2I5B.pdb: