Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKNIGCWLLLAGSSSVCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVALIDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTNPSFNIAIDTDAGGGNPFLNLAVTMGSDVNGIRLLPFSVYLAPSTSLPSPSDYALTSYAPKSIFSSGTTVNTGVKELIRSTGNLDINFVQTNKPRLNIQLGHAAQSVMVKFGGAIQSICSAASGCPITLVSDNTGATFGFKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKFDASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF 2FYZ Chain:F ((444-458)) ----------------------------------RGGIDISTELSKVNA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 2 115 57.25 7.63 target 2D structure prediction score : 0.07 Monomeric hydrophicity matching model chain F : 0.47 3D Compatibility (PKB) : 57.25 2D Compatibility (Sec. Struct. Predict.) : 0.07 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_2FYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FYZ-query.scw PDB file : Tito_Scwrl_2FYZ.pdb: