Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFGFTPSEGLLNDIQTGIANKNSSEPLYPLRDKIALQLNEEIIENVLTQLVQHFPASEKRDTAEKLAGYVKSTVAVLLKQLLSKAPNDVVKQSVEFSEKSLFKDPQGQYKVGVALDAGLVTNLKHN-----------F----AELQAGNDINKAALAELYKQFGDAMVRHFMSDFNKTLDLGMIKRKAADIGAAAVTKAVHIAIDKLIPSLNRTELKAMAEYHDGLFFN 3GA9 Chain:L ((134-203)) -----------------------------------------------------------------------------------------------------------------GFKVDDSLTMRLDLAKPRIYSDKLSIFYPNGEPIETGETLIQTDLARTLKKIQKEGAKGFYEGG------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 119 -1987 -16.69 -40.54 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain L : 0.57 3D Compatibility (PKB) : -16.69 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3GA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GA9-query.scw PDB file : Tito_Scwrl_3GA9.pdb: