Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATNYGTGRRKTATARVFLSAGTGKLVINNRTLEQYFGRETARMVVRQPLELLEATEKYDLYITVKGGGIGGQAGAIRHGITRALIAADETLKPVLRQAGFVTRDAREVERKKLGLRKARKRPQFSKR 5NO4 Chain:I ((4-127)) ----YGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR

General information: TITO was launched using: RESULT: Template: 5NO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 473 -47201 -99.79 -380.65 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain I : 0.95 3D Compatibility (PKB) : -99.79 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_5NO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5NO4-query.scw PDB file : Tito_Scwrl_5NO4.pdb: