Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPILRKLYKDD-DEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA 2JTT Chain:C ((192-212)) ------------------------------------------LMNVLKKIYEDGDDDMKRTIN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JTT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 5 315 62.90 15.73 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.42 3D Compatibility (PKB) : 62.90 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_2JTT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JTT-query.scw PDB file : Tito_Scwrl_2JTT.pdb: