Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKAHKNIWNKEKKTMNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFEGVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRALHGKAARIKEIRR 3J8G Chain:P ((1-111)) ----------------SNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKE---

General information: TITO was launched using: RESULT: Template: 3J8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 401 -24463 -61.00 -220.38 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain P : 0.82 3D Compatibility (PKB) : -61.00 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_3J8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J8G-query.scw PDB file : Tito_Scwrl_3J8G.pdb: