Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKDLYLFMKDIYQNRKLL--LQFSIND-FKSRYAGSFLGILWAFINPLFTVLI---------------YWLV---FGVG---------LKAQMIDAKYPFIVYLVTGMIPWFFFSDAF-----ASTTVVFREYTYLVKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAFLTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKL--NPLYYVVQGYRESFLSEAWFWEHGTYTLYF----WVFTLVMFVIGSAVFRRLKPHFSDVL 5FYP Chain:A ((2-298)) --QESPAFIDPASWNTPFNGIAQVACHNCYEKQYANTF----SSVLDSVRTLELDFWDQRDAVSGGSPHHWFVRHNPGSGNDNNCTKNDLEACLNDVKNWSDKH--PGHFPITLILDKKQGWSKESSGRTPKDFDELVARV-FQGKLFTPQDLATHIGSG----AGALQGNLKGKSWPTANDLQGKVLLVLNH---SENQKLSQYAEARTSKAKVFISPVTNGQNDISGKVSGMSSQSSGYVAMNNMGKGDKSWAKQAFAYSHIGRVWGDDEVSFAQHINQKINLSAYYRFAAQSAGGYRIRPF-----

General information: TITO was launched using: RESULT: Template: 5FYP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1353 -68553 -50.67 -277.54 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -50.67 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_5FYP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FYP-query.scw PDB file : Tito_Scwrl_5FYP.pdb: