Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHFAHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLAIEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVHCWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEKVSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLYRMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK 5K2M Chain:F ((20-34)) --------------------QIVECPVCGAELEVV---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5K2M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 18 -1493 -82.94 -99.53 target 2D structure prediction score : 0.07 Monomeric hydrophicity matching model chain F : 0.54 3D Compatibility (PKB) : -82.94 2D Compatibility (Sec. Struct. Predict.) : 0.07 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.719

(partial model without unconserved sides chains): PDB file : Tito_5K2M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K2M-query.scw PDB file : Tito_Scwrl_5K2M.pdb: