Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTAEKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQGGYTRILKTEPRRGDGAPMVVLELV 5MLC Chain:P ((19-126)) ----RLSRPADQRRALLRGLTTQLLKHGRIKTTRAKASAMRKYVDKMITLAKEGSLHKRRQALGFI----------YEKQIVHA----LFAEVPERYGDRNGGYTRIIRTLPRRGDNAPMAYIELV

General information: TITO was launched using: RESULT: Template: 5MLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 364 -47712 -131.08 -441.78 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain P : 0.85 3D Compatibility (PKB) : -131.08 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.695

(partial model without unconserved sides chains): PDB file : Tito_5MLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MLC-query.scw PDB file : Tito_Scwrl_5MLC.pdb: