Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTHNDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHK---Y-PNVKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSN-YSFIWAMCGENITYTDMDMVQMDQLK 1SEF Chain:A ((84-257)) ---------------------------------------------------------------------------IQTL-VYVIDGRLRVSDGQETHELEAGGYAYFTPEMKMYLAN-AQ-EADTEVFLYKKRYQPLAGHQPYKVVGSIHDQQPEEYEG---MTDVLLWSLLPKE-F-DFDMNMHILSFEPGA-S-HAYIETHV-QEHGAY--LIS---GQGMYNLD--NEW--YPVEKGDYIFMSAYVPQAAYAVGREEPLMYVYSKD----------------

General information: TITO was launched using: RESULT: Template: 1SEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 829 -11138 -13.44 -65.91 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -13.44 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_1SEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SEF-query.scw PDB file : Tito_Scwrl_1SEF.pdb: