Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRKDEHVSLAKAFHD----KQKNEFDFVRIIHNPLPQIAVSDVDLSTQAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADTYTIMRQEYPHGKIIANIG-----AGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFTNWKV-LIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENARRSKR------ELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGTSGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCEARGIDLVKYGRRTEKSVGQK 3B04 Chain:A ((6-361)) -NRKVEHVEIA-AFENVDGLSSSTFLNDVILVHQGFPGISFSEINTKTKFFRKEISVPVMVTGMTGGRNELGRINKIIAEVAEKFGIPMGVGSQRVAIEKAEARESFAIVRKVAPTIPIIANLGMPQLVKGYGLKEFQDAIQMIEADAIAVHLNPAQEVFQPEGEPEYQIYALEKLRDISKELSVPIIVKESGNGISMETAKLLYSYGIKNFDTSGQGGTNWIAIEMIRDIRRGNWKAESAKNFLDWGVPTAASIMEVRYSVPDSFLVGSGGIRSGLDAAKAIALGADIAGMALPVLKSAI-EGKESLEQFFRKIIFELKAAMMLTGSKDVDALKKTSIVILGKLKEWAEYRGINLSIY-----------

General information: TITO was launched using: RESULT: Template: 3B04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2075 59108 28.49 173.85 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 28.49 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3B04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B04-query.scw PDB file : Tito_Scwrl_3B04.pdb: