Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIID-SLPQLGGQLATLYPEKYIYDIPGYPAIKASELIDQLEKQLTTFN----HTFHLKEEVLSLTREDEVIEITTNKGIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYF-VNDLMKYAGKKVAIAGGGDSAIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSR-LKSSSVNLLTPYLIDGLSGNNGELTDIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGVYAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS 5MIP Chain:A ((25-323)) ---YDVVIIGSGPAGMTAAMYTARSEMKTLLLERGVP--GGQMNNTAE---IENYPGYETIMGPELSMKMAEPLEGLGVENAYGF-----VTGIEDHGDYKKIITEDDEFITKSIIIATGANH---RKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGITDEAGWIITDDHMRTNIPGIFAVGDV--RQKDFRQITTAVGDGAQAAQEAYKFV----------------------

General information: TITO was launched using: RESULT: Template: 5MIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1730 33105 19.14 113.37 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 19.14 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_5MIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MIP-query.scw PDB file : Tito_Scwrl_5MIP.pdb: