Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTFRLILDFLTQDQGEVLWNGHHLGEKDYDV-----IGYLPEERGLYPKVTIQDQLLYFAELRGKSRKEIEPKIDFWMEKF-QVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGLDPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRESFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYIPMFNQQPPTLEEIFKLKAGEIDA 1JI0 Chain:A ((6-237)) VLEVQSLHVYYGAIHAIKGIDLKVPRGQIVTLIGANGAGKTTTLSAIAGLVRAQKGKIIFNGQDITNKPAHVINRMGIALVPEGRRIFPELTVYENLMMGAYNR-KDKEGIKRDLEWIFSLFPRLKERLKQLGGTLSGGEQQMLAIGRALMSRPKLLMMDEPSLGLAPILVSEVFEVIQKINQEGTTILLVEQNALGALKVAHYGYVLETGQIVLEGKASELLDNEMVRKAYL------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1JI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1182 -11230 -9.50 -49.69 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -9.50 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1JI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JI0-query.scw PDB file : Tito_Scwrl_1JI0.pdb: