Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQGFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEASFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEKKD 5H7X Chain:E ((9-167)) MSKTRVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGHHKNPLFSLEERVALAQSSLGHLSNVEFVGF-DGLLVNFFKEQKATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKH--

General information: TITO was launched using: RESULT: Template: 5H7X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 740 -94948 -128.31 -597.16 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.87 3D Compatibility (PKB) : -128.31 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_5H7X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H7X-query.scw PDB file : Tito_Scwrl_5H7X.pdb: