Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKALPIAVAVVLGGAALVPVYYATQHPTTEVGHKADKNASPIQKISYVLGYEVAQQTPPE---LDTKAFVQGIHDVRNKQPSAYTQEDLKAAVAAYEKELQQKMQHQDKPEQAGTATDSADAQFLAENKTKAGVKTTASGLQYIITKEGTGKQPTAQSIVKVHYEGRLINGQVFDSSYKRGQPVEFPLNQVIPGWTEGLQLMKEGGKATFFIPSNLAYGPQELPG-IPANSTLIFDVELISVK-- 1FD9 Chain:A ((9-212)) ----------------------------------------TDKDKLSYSIGADLGKNFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD--ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINSGNGVKPGKSDTVTVEYTGRLIDGTVFDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGPNETLIFKIHLISVKKS

General information: TITO was launched using: RESULT: Template: 1FD9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 691 26179 37.88 132.21 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 37.88 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1FD9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FD9-query.scw PDB file : Tito_Scwrl_1FD9.pdb: