Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSTHRSKT--ICSKMARAFPGYSVMFFMHKKTLVLLLSTFCAS----TYIHAKTDCYPSSPPVVKQLDQVYYGMNNLRIFYTTSSDNALT-FQQDKNLNHIPDYVENIAIQANSIRKAWSVLGYQDPLNSPRYKNDVKYIDIQIQKLNGLGLAYDEPHRHINNPLKKNACALIILISNNI-PNLTTNSSLIAHELFHLYTYGYTMFKQPWLTESLAAWSEGIIRKDKLGIYGQRNLPENEEDFEKYVLQRTYRTSQLWNRLTTLIDRSDGKLFLPLSLM-QQKYTDGQPVFQ------DKYLK------------GTLFIRKFLE---NLGRQD-QKISVLNNWNLYHWNEIDQNSAHFNEQILDTLLITLESTVERNIEKNKFKNLLKKIVKNKKTN 5TF6 Chain:A ((1-367)) MLTRNRELTTVLVKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVV-GQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIR-----------LPSLRFNTSRRFAYIDVTSKEDARYCVEKL-----NGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGF--GSIEKINIP-AGQK-----EHSFNNCCAFMVFENKDSAERALQMNRSLLGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILN------GSQFQGKVIRSGTINDMKRYYNNQQN-

General information: TITO was launched using: RESULT: Template: 5TF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1480 95077 64.24 282.97 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 64.24 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_5TF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TF6-query.scw PDB file : Tito_Scwrl_5TF6.pdb: