Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSEEMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNGLGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGKFIDVYLMGKLIG 4R9M Chain:A ((28-190)) -AHSVKLRPLEREDLRYVHQLD---SVMRYWFEEP---YE-AFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINH--VHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFIN-GQYRNAIRMCIFQ-

General information: TITO was launched using: RESULT: Template: 4R9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 652 -96790 -148.45 -604.94 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -148.45 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_4R9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R9M-query.scw PDB file : Tito_Scwrl_4R9M.pdb: