Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIILASAMSGVVLLLGACATTPKNTLAIQKENNQFEVTGIGKTNLIAKNNAVTAAQKTCSKSTTPVIVDEKTSYHGVLKDVVSEKTGQMVEAAAGVIGTLTGKNASLAKDDDYQTTLTFYCKIN 2K8S Chain:A ((1-80)) --------VASKAIFYHAGCPVCVSAEQAVANAIDP-SKYTVEIVHLGTDKARIAEAEKA-GVKSVPALVIDGAAFHINFGAGIDDLKGS------------------------------------

General information: TITO was launched using: RESULT: Template: 2K8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 332 -32485 -97.84 -406.06 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -97.84 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_2K8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K8S-query.scw PDB file : Tito_Scwrl_2K8S.pdb: