Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKNIGGMKMIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGTTGESSTLTQSEEEQILQLTVKKVAG-RVPVIAGAGTNNTKETIEKAKHFASLGADALLVITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNL-AEHPNIIGLKEASGDMAYVMDAARLIGEE--FFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELVKDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDL-PSGPLRLPLVSLSKEKKKQLAQLLLKERTVEK 5F1V Chain:A ((2-293)) --KNI-----IIGAMTALITPF-KNGKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHRTCIEIAVETCKGTKVKVLAGAGSNATHEAVGLAKFAKEHGADGILSVAPYYNKPTQQGLYEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGV-EASGNIDKCVD---LLAHEPRMMLISGEDAINYPILSNGGKGVISVTSNLLPDMISALTHFALDENYKEAKKINDELYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLCSPSKENFAKIEEVMKKYK----

General information: TITO was launched using: RESULT: Template: 5F1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 -39860 -23.49 -139.37 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -23.49 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.650

(partial model without unconserved sides chains): PDB file : Tito_5F1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F1V-query.scw PDB file : Tito_Scwrl_5F1V.pdb: