Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFGAIGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLD-----GAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHY 1HCS Chain:B ((9-91)) WYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1HCS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 253 -5167 -20.42 -66.24 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -20.42 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_1HCS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HCS-query.scw PDB file : Tito_Scwrl_1HCS.pdb: