Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence YYHGRLTKQDCETLLLKEGVDGNFLLRDSESIPGVLCLCVSFKNIVYTYRIFREKHGYYRIQTAEGSPKQVFPSLKELISKF 4NWG Chain:A ((6-111)) WFHPNITGVEAENLLLTRGVDGSFLARPS--NPGDFTLSVR-RNGAVTHIKIQNTGDYYDLYGGEK-----FATLAELV---

General information: TITO was launched using: RESULT: Template: 4NWG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 -44991 -171.72 -633.67 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -171.72 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_4NWG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NWG-query.scw PDB file : Tito_Scwrl_4NWG.pdb: