Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAWQNVEVVHVVAMDKNHCIGKGNALPWHISADLKHFKEITQGGVVIMGRKTLESMGRALPNRVNWVITRDINWHFDGVKIAYSIEDALNAALEDAKNTEKQALFIIGGGEIFKQTLSIADRLELTHVDLDVQGDAHYPTIPSEFHKTAS--EQQVDEKSGTSFEFATYKK 3SRS Chain:X ((6-152)) ----------LVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLP---------GHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTF-----

General information: TITO was launched using: RESULT: Template: 3SRS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 613 -102231 -166.77 -705.04 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain X : 0.79 3D Compatibility (PKB) : -166.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_3SRS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SRS-query.scw PDB file : Tito_Scwrl_3SRS.pdb: