Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHIERESMEFDVVIVGAGPAGLSAAIKIRQLAIENNLPDLSVCVVEKGSEVGAHILSGAVLEPHAINELFPNWKEEGAPLNVPVTEDKTFFLLSETTSKEAPHWMVPK-TMYNDGNYVISLGNVVRWLGQKAEELEVSIFPGFAASEVLYHENGTVKGIQTGDMGIGKDGEPTHNFTPGYELHAKYTLFAEGCRGHLGKRLIAKYNLDKDADPQHYGIGIKELWEIDPAKHKPGLVMHGAGWPLSETGSS-GGWWLYHAEN--NQVTLGMIVDLSYENPHMYPFMEMQRWKTHPLIKQYLEGGKRISYGARAVVKGGFNSLPKLTFPGGCLIGDDAGFLNFAKIKGSHTAMKSGMLCGEAVFEAIAAGVEKGGDLAVARVTKGEDLFVK-ELTSYTEKFNNSWLKEELYNARNFGPAMHK-FGQWIGGAFNFIDQNV--FKVPFTLHDLKQDFAVLKTVDATSFKPTYPKPDGKLTFDRLSSVFISNTVHEENQPAHLKLTDPSIPVNVNLPKWDEPAQRYCPAGVYEIMEND-DGSKRFQINAANCVHCKTCDIKDPSQNITWVTPEGGGGPNYPNM 2GMJ Chain:A ((28-584)) --NMERFAEEADVVIVGAGPAGLSAATRLKQLAAQHEK-DLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGAPLNTPVTEDRFGILTE-KYRIPVPI--LPGLPMNNHGNYVVRLGHLVSWMGEQAEALGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKWKPGRVDHTVGWPLDR--HTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSPFREFQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGTLAAESIFNQLTSEN-------------LQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTLKHKGSDSDQLKPAK-DCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

General information: TITO was launched using: RESULT: Template: 2GMJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3367 -2942 -0.87 -5.37 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -0.87 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2GMJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GMJ-query.scw PDB file : Tito_Scwrl_2GMJ.pdb: