Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNLPIIAVLYMILSMVSYQISASFAKQLIAALDPLTVTILRLCFAAVIVAVMFRSWKIWSRLPYLKWRDLLMYCAS-LGLMNILFYTSLGKLPQGIAVGLEFIGPLGLALVSI----K--QKSEYIWVAFAMLGIALMV-PWQDP-TQHHFSYVGAACALGAGLCWAFYIYFGQKVVTQNIGMHALTIGITVSALALLPIGIWYNGPALLDTQYWGKALLIAVLATAIPYALDLMALKQLNKLTYGTLTSLAPALAALTGFLLLHEEISMLQWVALACVMLASIGVTLRSKTAS 5I20 Chain:E ((2-287)) ---SRSSATLIGFTAILLWSTLALATS-STGAVPPFLLTALTFTIGGAVGIAAGLARGVGLSVLRQPWPV-WVHGIGGLFGYHFFYFSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGARAGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFAR-VPTEVVAGFCLATAALSALCHILFEP-SVWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLLARR--

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1360 -208658 -153.43 -753.28 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.70 3D Compatibility (PKB) : -153.43 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: