Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNVLKHFKEKQMPSFQSADAQINYQTFGEPSSPALVFSNSLGTNYGMWQKQFNELKDQFFVICYDNRGHGSSSTPDGPYTVEQLGEDVVRLLDHLNISKAAFCGISMGGLTGQWLAIHYPNRFSHVVVANTAAKIGQEQAWLDRAKLVREQGLQPIATTAASRWFTDPFIQSHPSIVNNLCNDLSAG--SAMGYAN----CCEALAKADVREQLKDIKIPVLVIAGPQDPVTTVADGEFMQQRIPQSKLAEID-ASHISNVEQPEVFNKILKDFLVN 5EGN Chain:H ((2-259)) -------------PHVENDGVKIYYDSYG--EGVPIVFLHPFSTNGGIWYFQTFPFAQTNHVIVIDHRGHGRSDKPATGYSIMEHADDVVAVLDALKVDRAVFVGNSIGGMIAMQLNLDHPQRVIGNLILSSGTGLGEGMPPEAG-AAFQNDYIGAF-GGLLEGAVSARSKRERPEILAVMKAHFSVPSNFPKHVFDAATADPNGVFAWNIKDRLSSIQAPTLVVAGEEDLVTTVANNQLLADNIPGAELRVINDVGHFYQLERPSEFNELLRGF---

General information: TITO was launched using: RESULT: Template: 5EGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1477 -69343 -46.95 -276.27 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain H : 0.75 3D Compatibility (PKB) : -46.95 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_5EGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGN-query.scw PDB file : Tito_Scwrl_5EGN.pdb: