Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MYSTHRSKTICSKMARAFPGYSVMFFMHKKTLVLLLSTFC--ASTYIHAKTDCYPSSPPVVKQLDQVYYGMNNLRIFYTTSSDNALTFQQDKNLNHIPDYVENIAIQANSIRKAWSVLGYQDPLNSPRYKNDVKYIDIQIQKLNGLGLAYDEPHRHINNPLKKNACALIILISNNIPNLTTNSSLIAHELFHLYTYGYTMFKQPWLTESLAAWSEGIIRKDKLGIYGQRNLPENEEDFEKYVLQRTYRTSQLWNRL------TTLIDRSDGKLFLPLSLMQQKYTDGQPVF---QDKYLKGTLFIRKFLENLGRQDQKISVLNNWNLYHWNEIDQNSAHFNEQILDTL--LITLESTVERNIEKNKFKNLLKKIVKNKKTN- 5BZ1 Chain:A ((3-399)) VEISALPLRDLDYIKLATDQFGCRFLQKKLETPSESNMVRDLMYEQIKPFFLDLILDPFGNYLVQKLCDYLTAEQKTLLIQTIYPNVFQISI----------NQYGTRSLQKIIDTVDN-EVQIDLIIKGFS-----------QEFTSIEQVVTLINDLNGNHVIQKCIFKF--SPSKFGFIIDAIVEQNNIITISTHKH--GCCVLQKLLSVCTLQQIFKISVKIVQFLPGLIN-DQFGNYIIQFLLDIKELDFYLLAELFNRLSNELCQLSCLKFSSNVVEKFIKKLFRIITGFIVNNVASDDVINASMNILLTTIDIFTVNLNVLIRDNFGNYALQTLLDVKNYSPLLNYGNFCNDFSLKIGNLIVLTKELLPSIKTTSYAKKIKLKVKAYAEAT

General information: TITO was launched using: RESULT: Template: 5BZ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1596 16269 10.19 47.71 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 10.19 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_5BZ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BZ1-query.scw PDB file : Tito_Scwrl_5BZ1.pdb: