Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKPLLLVFHPDLSQSKVNRALVATLKDQDKIDIVDIQKLY-NGSLDIMNDGAIEAQRLISASHIILQFPIYWYSVPPIFKAWMDAVLTRMFYLFYEQEGQHIEGKPLYLHITVGNLSSAYQKLGQNGFTLDELMAPLKATARRCGLIWHPPHVIFNANQLNENDITEQTSLSLKQFEQWGIFNNNSFILVLSQKKRTSEKSEVRTML 5LVA Chain:B ((3-161)) ---VLVLAFHPNMEQSVVNRAFADTLKDAPGITLRDLYQEYPD----EAIDVEKEQKLCEEHDRIVFQFPLYWYSSPPLLKKWLDHVLLYGWAYGT--NGTALRGKEFMVAVSAGAPEEAYQAGGSNHYAISELLRPFQATSNFIGTTYLPPYVFYQAGTAGKSELAE----------------------------------------

General information: TITO was launched using: RESULT: Template: 5LVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 696 -67031 -96.31 -424.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -96.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_5LVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LVA-query.scw PDB file : Tito_Scwrl_5LVA.pdb: