Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDVKKHPIGHWGTIPGQNFI-YVHLNRVIN-------KYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEIYPEVTEDETGMQKLFKRFSFPGGIASHAAP-ETPGSIHEGGELGYSLSHAVGAVLDN-----------------PEVISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDGELANYFNGLGWEPFFI-EGNDSEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPKWPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEKLFDAQGRILEEIREIAPTG---DQRMA----KN--PITNGGIDPRPL---IMP-DWKKYTLQFEKPGSVKAEDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLSPSGRVIDSQLSEHQDEGFLEGYVLTGR-HGFLQVTNHSCEWLTLC 3RIM Chain:A ((28-471)) ---------------------------------DTIRVLAADAVQKVGNGHPGTAMSLAPLAYTLFQRTMRHDPSDTHWLGRDRF-VLSAGHSSLTLYIQLYLGGFG------LELSD--IES-LRTWGS--KTPGHPEFRHTPGVEITTGPLGQGLASAVGMAMASRYERGLFDPDAEPGASPFDHYIYVIASDGDIEEG---VTSEASSLAAVQQLGNLIVFYDRNQISIEDDTNIA--LCEDTAARYRAYGWHVQEVEGGENVVG--------IEEAIANAQA------V-------TDRPSFIALRTVIGYPAPNL------MDTGKAHGAALGD-----DEVAAVKKIV-GFDPDKTFQVREDVLTHTRGLVARGKQAHERWQLEFDAWARREPERKALLDRLLAQKLPDGWDAD-LPHWEPGSKALATRAASGAVLSALGPKLPE-LWGGSADLAGSNNTTIKGA-D--SFGPPSISTKEYTAHWYGRTLHFGVREHAMGAILSGIVLHGPTRAYGGTFLQFSDYM---

General information: TITO was launched using: RESULT: Template: 3RIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2060 13245 6.43 32.86 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.43 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_3RIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RIM-query.scw PDB file : Tito_Scwrl_3RIM.pdb: