Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSLHRYKGKYMSNKRIAIIGAGPAGLMAAEVLSQYAYEIDVFEQKPSAARKFLMAGKTGLNISHAEPLPQFIERYDH-TEWLKPWVEKWDAVWIQKWMKSLGIESYVGTSGRIFPVEMKAAPLLRAWLKRLAEQQVKFFYRHRCINLQGTT---VTLENQNNQSTETFTQQYDAVILACGAVSWSQLGSDGAWQQWMDQS--SIEPFQASNAGVLHTWSAFMES-CF-GEPLKRINAWV-EPS-QVTHGDIVITHYGLESGVIYKLGRALREQQKQG--KSLNLFLDLLPDVELTQLVKKLQAS-K---KQSLTNIWRKAGL-DTAKINLIREV-VD--K--AL--WSQPETMAQKMKALQIKLDGFRPIEEAISCAGGVKQDALTESLE-LKQTSGVFCCGEMLDWDAPTGGYLLTACFATGRAAGEGVHSFLEK 2I0Z Chain:A ((25-444)) ------------MHYDVIVIGGGPSGLMAAIGAAEEGANVLLLDKGNKLGRKLAISGGGRCNVTNRLPLDEIVKHIPGNGRFLYSAFSIFNNEDIITFFENLGVKLKEEDHGRMFPVSNKAQSVVDALLTRLKDLGVKIRTNTPVETIEYENGQTKAVILQT---G--EVLETNHVVIAVGGKSVPQTGSTGDGYAWAEKAGHTITELFPTEVPILSNEP-FIRDRSLQGLALRDINLSVLN-AIISHKMDMLFTHFGLSGPAALRCSQFVVKALKKFKTNTIQMSIDALPEENSEQLFQRMLKQMKEDPKKGIKNVLKGY-VPERYFLFLLEKNEIDGSEQAGQVSHEKIRALVKDFKEFTVNVNGTQSIEKAFVTGGGVSVKEINPKEMSSKFTNGLYFCGEVLDIHGYTGGYNITSALVTGRIAGTTAGENAK-

General information: TITO was launched using: RESULT: Template: 2I0Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2120 -9309 -4.39 -23.81 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -4.39 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2I0Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I0Z-query.scw PDB file : Tito_Scwrl_2I0Z.pdb: