Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHSVALNFADGKTFFISVNKDELLLDAAVRQGINLPLDCREGVCGTCQGQCETGIYEQEYVDEDALSERDLAERKMLACQTRVKSDAAFYFDHDSAICNAGDTLKIETQVTAVELVSETTAILHLDASNHAEQLQFLPGQYARLQIPD--TEDWRSYSFANRPNATNQLQFLIRLLPDGVMSNYLRDRCQVGQSLLIE-APLGSFYLRE--VERPLVFVAGGTGLSAFLGMLDNLVEQPNSPTVQFYYGVNSETDLCEQQRLHAYAEQLPN--------FSYHPIVTKATETWQGK------AGYIHEHLNKDQLAEQAFDMYLCGPPPMIEAVKNWLDEQALQNY-------RIYSEKFLQSNTSR 2QDX Chain:A ((3-256)) ---------------------------------------------------------------------------------------------------------LYTERVLSVHHWNDTLFSFKTTRN---PGLRFKTGQFVMIGLEVDGRPLMRAYSIASPNY-EEHLEFFSIKVPDGPLTSRLQH-LKEGDELMVSRKPTGTLVHDDLLPGKHLYLLSTGTGMAPFLSVIQDPETYERYEKVILVHGVRWVSELAYADFITKVLPEHEYFGDQVKEKLIYYPLVTREPFRNQGRQTDLMRSGKLFEDIGLPPMNPQDDRAMICGSPSMLEETSAVLDSFGLKISPRMGEPGDYLIERAFVE----

General information: TITO was launched using: RESULT: Template: 2QDX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 -14700 -12.16 -64.47 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -12.16 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2QDX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QDX-query.scw PDB file : Tito_Scwrl_2QDX.pdb: