Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHIERESMEFDVVIVGAGPAGLSAAIKIRQLAIENNLPDLSVCVVEKGSEVGAHILSGAVLEPHAINELFPNWKEEGAPLNVPVTEDKTFFLLSETTSKEAPHWMVPK-TMYNDGNYVISLGNVVRWLGQKAEELEVSIFPGFAASEVLYHENGTVKGIQTGDMGIGKDGEPTHNFTPGYELHAKYTLFAEGCRGHLGKRLIAKYNLDKDADPQHYGIGIKELWEIDPAKHKPGLVMHGAGWPLSETGSS-GGWWLYHAEN--NQVTLGMIVDLSYENPHMYPFMEMQRWKTHPLIKQYLEGGKRISYGARAVVKGGFNSLPKLTFPGGCLIGDDAGFLNFAKIKGSHTAMKSGMLCGEAVFEAIAAGVEKGGDLAVARVTKGEDLFVK-ELTSYTEKFNNSWLKEELYNARNFGPAMHK-FGQWIGGAFNFIDQNV--FKVPFTLHDLKQDFAVLKTVDATSFKPTYPKPDGKLTFDRLSSVFISNTVHEENQPAHLKLTDPSIPVNVNLPKWDEPAQRYCPAGVYEIMEND-DGSKRFQINAANCVHCKTCDIKDPSQNITWVTPEGGGGPNYPNM 2GMH Chain:B ((28-584)) --NMERFAEEADVVIVGAGPAGLSAATRLKQLAAQHEK-DLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGAPLNTPVTEDRFGILTE-KYRIPVPI--LPGLPMNNHGNYVVRLGHLVSWMGEQAEALGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKWKPGRVDHTVGWPLDR--HTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSPFREFQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGTLAAESIFNQLTSEN-------------LQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTLKHKGSDSDQLKPAK-DCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

General information: TITO was launched using: RESULT: Template: 2GMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3387 2681 0.79 4.89 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 0.79 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2GMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GMH-query.scw PDB file : Tito_Scwrl_2GMH.pdb: