Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNLNLNINQNDVELIPENHPESQEPVVGAEILIEQLYKFYGEVKVLEDLNLHIQPGEFLAIVGRSGCGKSTLLRLIAQLEKASFGEI-------KFKSARHLREGITNDDIRVMFQDPRLLPWRNILQNVQLGLP-------------KSQHALAEELLEKVGLKEKAGQWPSQLSGGQRQRTALARALSHTPRILLLDEPLGALDALTRLEMQSLIERLWKEQGFTAILVTHDVSEAVQLADRIILLDKGQIAQSFQVNLPRPRKKSITFAQLEQQVLDAVLAT 2FGJ Chain:C ((24-223)) ---------------------------------------------ILDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPNWLRR-----QVGVVLQDNVLLN-RSIIDNISLANPGMSVEKVIYAAKLAGAHDFISELRE--GYNTIVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALDYESEHVIMRNMHKICK--GRTVIIIAARLS-TVKNADRIIVMEKGKIVE------------------------------

General information: TITO was launched using: RESULT: Template: 2FGJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 794 -1770 -2.23 -9.83 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -2.23 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_2FGJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FGJ-query.scw PDB file : Tito_Scwrl_2FGJ.pdb: