Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNVLKHFKEKQMPSFQSADAQINYQTFGEPSSPALVFSNSLGTNYGMWQKQFNELKDQFFVICYDNRGHGSSSTPD--GPYTVEQLGEDVVRLLDHLNISKAAFCGISMGGLTGQWLAIHYPNRFSHVVVANTAAKIGQEQAWLDR-----AKLVRE----QGLQPIATT----AASRWFT-DPFIQ--SHPSIVNNLCNDLSAGSAMG---YANCCEALAKADVR------EQLKDIKIPVLVIAGPQDPVTTVADGEFMQQRIPQSKLAEID-ASHISNVEQPEVFNKILKDFLVN 4INZ Chain:B ((21-304)) -------------QYINVNGVNLHYISKG--QGELMLFLHGFPDFSHIWRHQIDEFSNDFHTVALDLRGYNLSEKPSGLESYEIDVLVEDIRQVIEGLGYSSCTLVVHDWGAGIGWTFAYRYPEYVQKLIAFNGPHPYTFMRELRTNKNQQKASEYAKWFQKQEVQDYMERDNFSGLRKLVIDPGVKKGYLTADDVQAYMNSWENGSVLSMLSYYRNLKIFTEEDLRRKSLFPLEEEVLNIPVQIIWGNQDPTFMPENLDGIEEYVPNISVHRLAEASHAPQHEKPQEVNNVMWNFLNK

General information: TITO was launched using: RESULT: Template: 4INZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1334 -39396 -29.53 -153.89 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -29.53 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_4INZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4INZ-query.scw PDB file : Tito_Scwrl_4INZ.pdb: