Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTR-EPGGTPLAEQIRSMLLAVNHDENMSHDTELLLIYAARAQHLQQVILPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFV-SRMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFFTKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA 3LV8 Chain:A ((28-226)) KFIVIEGLEGAGKSTAIQVVVETLQQNGIDHITRTREPGGTLLAEKLRALVKEEHPGEELQDITELLLVYAARVQLVENVIKPALARGEWVVGDRHDMSSQAYQGGGRQIAPSTMQSLKQTALGDFKPDLTLYLDIDPKLGLER-----ELDRIEKMDISFFERARERYLELANSD-DSVVMIDAAQSIEQVTADIRRAL-

General information: TITO was launched using: RESULT: Template: 3LV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 904 19864 21.97 103.46 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 21.97 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3LV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LV8-query.scw PDB file : Tito_Scwrl_3LV8.pdb: