Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MKVFDYEDIQLIPNKCIVN-SRSECDTTVTLGKH--TFKMPVVPANMQTIIDETIAEFLAENGYFYIMHR-FDEAARIPFIKKMK---------KRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARK---PIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQHKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRK-VDYVIVKNSIFNGDTI 1B3O Chain:A ((10-498)) TSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYID--FTADQVDLTSALTK-KITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGV--DVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMG---------------SRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGE-----------------------------------DKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQ

General information: TITO was launched using: RESULT: Template: 1B3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1538 44465 28.91 164.68 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 28.91 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_1B3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B3O-query.scw PDB file : Tito_Scwrl_1B3O.pdb: