Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEYELLIREAEPKDAAELVAFLNRVSLE-TDFTSLDGDGILLTSEEMEIFLNKQA---SSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNGLGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGKFIDVYLMGKLIG 3R9F Chain:B ((19-187)) -NDEITLLYPALKYAEELYLLINQNKINFIKSMAWPA--FVNNISDSVSFIEQSMIDNQNEKALILFIKYKTKIAGVVSFNIIDH--ANKTAYIGYWLGANFQGKGIVTNAINKLIQEYGDSGVIKRFVIKCIVDNKKSNATALRCGFTLEGVLQKAEILN-GVSYDQNIYSKVI-

General information: TITO was launched using: RESULT: Template: 3R9F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 697 -32776 -47.02 -198.64 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -47.02 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_3R9F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R9F-query.scw PDB file : Tito_Scwrl_3R9F.pdb: