Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISFEAGINQFGGSAIFLSPRDTQLGRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKVPVINGLTDDHHPCQLLADLQTYIEYRG--SIEGKTVAWIGDGN-NMCNSYIEAAHMMGFKLKIASPKGYEPKPEFLA-------EFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQ-KLREKAFADFQVNEKLMGL-AHPDCLFMHCLPA------------------HRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLNENLKKA 1DUV Chain:H ((3-332)) FYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLTGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGYGQEIVETLAEYASVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTEDVAKGVEGADFIYTDVWVSMGEAKEKWAERIALLREYQVNSKMMQLTGNPEVKFLHCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLS------

General information: TITO was launched using: RESULT: Template: 1DUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1597 -16358 -10.24 -54.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain H : 0.80 3D Compatibility (PKB) : -10.24 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_1DUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DUV-query.scw PDB file : Tito_Scwrl_1DUV.pdb: