Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGRKTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSRIFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIE-E-YEGITDQENLYAHRFQKWERVKNRVASKEE 3FYV Chain:X ((1-158)) TLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIG-KPLPNRRNVVLTSDTSFN-VEGVDVIHSIEDIYQL----PGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKL--DEKNTIPHTFLHLIRKK--------

General information: TITO was launched using: RESULT: Template: 3FYV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 755 -110988 -147.00 -711.46 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain X : 0.79 3D Compatibility (PKB) : -147.00 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_3FYV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FYV-query.scw PDB file : Tito_Scwrl_3FYV.pdb: