Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEGQWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIEAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE 1IOW Chain:? ((1-96)) MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPV----------------------------DPKEVDVTQLK---------SMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMD-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1IOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 434 -6209 -14.31 -66.05 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -14.31 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1IOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IOW-query.scw PDB file : Tito_Scwrl_1IOW.pdb: