Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSLHRYKGKYMSNKRIAIIGAGPAGLMAAEVLSQYAYEIDVFEQKPSAARKFLMAGKTGLNISHAEPLPQFIERY-DH-TEWLKPWVEKWDAVWIQKWMKSLGIESYVGTSGRIFPVEMKAAPLLRAWLKRLAEQQVKFFYRHRCINLQGT---T-VTLENQ-NNQSTETFTQQYDAVILACGAVSWSQLGSDGAWQQWMDQS--SIEPFQASNAGVLHTWS-AFMESCF-GEPLKRINAWVEPSQVTHGDIVITHYGLESGVIYKLGRALREQQKQGKSLNLFLDLLPDVEL-TQLVKKLQASKKQSLTNIWRKAGLDTAKINLIREVV-DK---AL--WSQPETMAQKMKALQIKLDGFRPIEEAISCAGGVKQDALTESLE-LKQTSGVFCCGEMLDWDAPTGGYLLTACFATGRAAGEGVHSFLEK 2GQF Chain:A ((2-401)) -----------SQYSENIIIGAGAAGLFCAAQLAKLGKSVTVFDNGKKIGRKILMSGGGFCNFTNLEVTP---AHYLSQNPHFVKSALARYTNWDFISLVAEQGITYHEKELGQLFCDE-GAEQIVEMLKSECDKYGAKILLRSEVSQVERIQNDEKVRFVLQVN---S--TQWQCKNLIVATGGLSMPGLGATPFGYQIAEQFGIPVIPPRASLVPFTYRETDKFLT-ALSGISL-PVTITALCGKSFYNQLLFTHRGISGPAVLQISNYWQPT------ESVEIDLLPNHNVEEEINQAKQSSPKQMLKTILVRLLPKKLVELWIEQGIVQDEVIANISKVRVKNLVDFIHHWEFTPNGTEGYRTAEVTMGGVDTKVISSKTMESNQVSGLYFIGEVLDVTGWLGGYNFQWAWSSAYACALSISRQ---

General information: TITO was launched using: RESULT: Template: 2GQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2095 -44091 -21.05 -115.72 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -21.05 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_2GQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GQF-query.scw PDB file : Tito_Scwrl_2GQF.pdb: